Cardiovascular diseases (CVD) constitute the leading cause of death worldwide. Not only is it affecting quality of life for these patients, but it also puts a huge economic burden on society. This increasing trend of CVD provides evidence that more research needs to be done to obtain more insight into the molecular mechanisms of these cardiac disorders. Furthermore, conducting research on relatively rare diseases such as Marfan syndrome (MFS) is an important contributor to raising awareness to these patients. In this master’s dissertation, we tried to contribute to the construction of a physiological relevant in vitro disease model for MFS. Generation of a fully optimized and characterized 3D in vitro disease model from induced pluripotent stem cells is beneficial due to its patient-specificity. It is a promising tool for narrowing the gap between native tissues and in vitro tissue models. They can be used for reliable studying of MFS, extensive drug screening, and progression towards personalized medicine. Newly gained information could contribute to treatment optimization, increasing the life expectancy and improving the quality of life for these patients.