Head and neck squamous cell carcinoma (HNSCC) is the most frequent tumour appearing in the head and neck region. It is the sixth most common cancer worldwide and the number of patients is increasing. Possible risk factors to develop HNSCC are smoking and alcohol consumption. Also oral infection with the human papillomavirus (HPV), mainly known by the public as a risk factor to get cervical cancer, is associated with HNSCC development. During the transformation of normal cells into HNSCC cells, specific genetic changes in the DNA of the cell occur. These changes allow the cell to progressively adopt tumour characteristics, such as faster cell growth and cell division. The progression into cancer happens gradually as the cell acquires more and more mutations leading to more and more tumorous characteristics. Recent research revealed that cells in normal tissues carry plenty of these specific genetic changes. Thus, these mutations are not only present in cancer cells. Interestingly, each cell that has such a specific genetic mutation forms a clone of identical cells due to repeated cell division. Normal tissues get completely occupied by normal appearing clones of cells, whose existence is driven by the same genetic mutations that also appear to cause cancer. Unravelling this clonal landscape can contribute to insights in cancer development and probably has imported consequences for cancer prevention, diagnosis and therapy. Previous research on skin tissue, oesophageal tissue and liver tissue for instance, already proved the presence of these changes. In head and neck tissue however, the mutational landscape has not yet been explored. To discover the mutational landscape in head and neck tissue, we developed a head and neck biobank with normal tissues using whole-body donors. A biopsy is taken from each donor eligible for our biobank from six different locations. The locations are specifically chosen as the six most frequent HNSCC locations. In total, 30 donors will be included in the biobank from which 15 are smokers and 15 are non-smokers. In the future, procedures like DNA sequencing will help in exploring the mutational landscape in the head and neck region. For this project, we rely heavily on the generous individuals who have chosen to selflessly donate their bodies to science. Above all, we wish to extend our deepest admiration and gratitude to these donors and their families for their essential role in not only our master’s thesis project, but also in medical research in general.